Optimization and Improvement of Emulsion PCR for the Ion Torrent Next-Generation Sequencing Platform
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Rapid flow-sorting to simultaneously resolve multiplex massively parallel sequencing products
Sample preparation for Roche/454, ABI/SOLiD and Life Technologies/Ion Torrent sequencing are based on amplification of library fragments on the surface of beads prior to sequencing. Commonly, libraries are barcoded and pooled, to maximise the sequence output of each sequence run. Here, we describe a novel approach for normalization of multiplex next generation sequencing libraries after emulsio...
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تاریخ انتشار 2011